MOLECULAR CHARACTERIZATION, ANTIMICROBIAL AND LARVICIDAL POTENTIALS OF FUNGI ISOLATED FROM SOIL SAMPLES OF LAUTECH BOTANICAL GARDEN OGBOMOSO, NIGERIA.
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The menace of malaria infection has necessitated the need for an eradication strategy either directly against Plasmodium or indirectly against its host, the mosquito. Eradication of mosquito can be more easily achieved by attacking the larva stage, this require the availability of an effective larvicide. This research work is focused on the production of biologically synthesized larvicide by exploring microorganisms isolated from the environment. In this study, soil fungi were isolated and inoculated on Potato Dextrose plate. Pure colonies were collected and identified by morphological and molecular characterization. The evolutionary relatedness was established using bioinformatics tools. The fungi colonies were subjected to submerged fermentation in Potato Dextrose broth and incubated for 21 days in a rotary shaker incubator for the production of the intracellularly metabolites. The metabolites were extracted and varying concentrations were used in challenging mosquito larva. Time of action and lethal dose (LD50) of each fungal metabolite were determined. Fourier Transform Infrared (FTIR) was used to monitor the bioactive compounds in the metabolites at the range of 4000-500 cm-1with an IR Affinity-1 Shimadzu Spectrometer. The results showed that metabolites of Penicillum chrysogenum, Aspergillus aculeatus, Talaromyces albobiverticillus and Aspergillus carbonarius possess potent larvicidal activities against the mosquito larvae at different concentrations and duration of lethal action. The band of the biomolecules present monitored with FTIR showed that they possesses: (C-O), (C≡C), (C≡N), (C-C=C), ((N-H), (OH), (C-I), (H-C-H), (N=O) and aromatic rings.
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Authors: Olusola Nathaniel Majolagbe,
Daniel Ayandiran Aina,
Louis Ezediuno, Olusola Ogunmodede, Aminat Kehinde, Blessing Oguntuase, Adebimpe Adio, Amos Thomas, Nafisat Kolawole, Esther Makanjuola, Grace Adebayo.
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Keywords: Soil fungi, Metabolite, larvicidal, FTIR, bioinformatics tools
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