DETECTION AND PARTIAL NUCLEOTIDE SEQUENCE OF EGG DROP SYNDROME VIRUS FROM A POULTRY FLOCK IN OGUN STATE, NIGERIA
|
This study was carried out based on a case of Egg Drop Syndrome (EDS) which occurred on a poultry farm at Ayepe Road, Shagamu, Ogun State, Nigeria which was investigated using molecular methods. Uterine tissue samples were collected from five randomly selected birds from the affected flock. Tissues and EDS Virus obtained from the Virus Research Unit of the National Veterinary Research Institute, Vom, Nigeria which served as positive control, were subjected to viral DNA extraction using QIA amp DNA mini kit and DNA blood mini kit 02/2003.
A Polymerase Chain Reaction (PCR) protocol was optimized and used for the amplification of a 238bp fragment of proteinVIII (pVIII) gene of EDS Virus (EDSV) from both the positive control and test DNA. The 238bp amplicon was partially sequenced using the Big Dye terminator method on ABI prism automatic sequencer. The partial nucleotide sequence of pviii gene was generated. The result obtained shows that nucleotide sequence of test DNA and those of EDSV and Duck Adenovirus Virus obtained from European Molecular Biology laboratories (EMBL) data base with accession No. Y09598 and NC-001813 both had 100% homology respectively. A Guanine-Cytosine content of 41.59% was also found. The region sequenced acts as a signal for late protein synthesis, thereby serving as confirmatory diagnosis in persistent EDSV infection. This study has established the preliminary detection and sequencing of the EDSV genome as well as the best optimization condition involved in a PCR protocol for rapid diagnosis of EDSV in Nigeria.
Also, the result from our study suggested that the regional isolation and determination of the genetic differences of EDSV in Nigeria is highly recommended.
|
Authors: Ockiya, Michael,A.;
Jarikpe, Theophilus. A;
Jibril, Afusat Jagun T;
Adeyefa, C.A.O
|
Keywords: Egg Drop Syndrome Virus, Protein VIII, Partial Nucleotide Sequence, Avian Adenovirus, Ayepe-Sagamu, Ogun State
|
|